Development and validation of RP-HPLC method for simultaneous estimation of paracetamol and chlorzoxazone in bulk form

Authors

  • Hajera N. Khan Department of Quality Assurance, Indira college of Pharmacy, Vishnupuri, Nanded, Maharashtra, India.
  • Mahajan Swarali Department of Quality Assurance, Indira college of Pharmacy, Vishnupuri, Nanded, Maharashtra, India.
  • Chopde Asha Department of Quality Assurance, Indira college of Pharmacy, Vishnupuri, Nanded, Maharashtra, India.
  • Mohammad Zameeruddin Department of Quality Assurance, Indira college of Pharmacy, Vishnupuri, Nanded, Maharashtra, India.
  • Vishvanath B. Bharkad Department of Quality Assurance, Indira college of Pharmacy, Vishnupuri, Nanded, Maharashtra, India.

Keywords:

RP-HPLC, Paracetamol, Chlorzoxazone, Validation

Abstract

High performance liquid chromatography (HPLC) method was developed and validated for the analysis of Paracetamol and Chlorzoxazone. Chromatographic separation achieved isocretically on C -18 column Zorbax Eclipse XDB- C18 (4.6~250mm~5µ). Utilizing a mobile phase Acetonitrile: Water in the ratio 50:50v/v. with a flow  rate  of  1.5ml/min.  UV  detection  was  carried  o ut  at  274nm.  The  retention time  of  Paracetamol  and Chlorzoxazone <10 min respectively. The developed method was validated in terms of recovery, precision,,rggedness, robustness, linearity as per ICH guidelines. This study aimed at developing and validating an HPLC method.

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Published

2022-10-11

Issue

Vol. 6 No. 3 (2017): 2017 Volume - 6 Issue - 3

Section

Articles