Validation of UV spectrophotometric method for the analysis of capsaicin in ethanol

Abstract

Method validation of gift sample capsaicin was carried out in calibrated double beam UV/visible spectrophotometer (Shimazdu UV-01800) using ethanol as diluent phase. Though, Capsaicin showed maximum absorbance peaks at 227nm and 280 nm, a 280nm wavelength was considered as absorbance peak as it was very strong at this wavelength. The correlation coefficient (R²) and linear regression equation were found to be 0.9924 and Y= 0.0155x -0.0338 respectively in linear- calibration curve. Further, this method was validated in terms of Linearity, Accuracy, Precision, Limit of Detection (LOD), Limit of Quantification (LOQ), repeatability and robustness as per ICH guidelines.